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Immunohistochemistry (IHC)
Immunohistochemistry (IHC) is a technique that utilizes antibodies that specifically recognize and bind different antigens in cells or tissue samples. The technique uses antibodies that are either conjugated to enzymes or a fluorescent dye to allow visualization of the distribution and localization of specific cellular components under a microscope.
The table below lists some examples of the cells which can be determined through CD markers via IHC across our facilities worldwide.
Immune Cells (CD45*) |
Species |
||||||
|---|---|---|---|---|---|---|---|
NHP |
Mouse |
Rat |
Porcine |
Canine |
Human |
||
Lymphoid |
T lymphocytes |
CD3, CD4, CD8, CD16, CD278 |
CD3, CD4, CD8 |
CD3, CD8 |
CD3, CD8 |
CD3, CD4, CD8 |
CD3, CD4, CD8 |
B lymphocytes |
CD19, CD20, CD21, CD79a, CD138 |
CD19, CD20, CD79a, CD45R |
CD79a |
CD79a |
CD20 |
CD20 |
|
NK cells |
CD8, CD16 |
CD8 |
CD8 |
CD8 |
CD8 |
CD8 |
|
Myeloid (Macrophages, Dendritic cells, Mast cells, Granulocytes) |
CD68, CD4, CD16, CD123, CD205, CD207, CD209, CD303 |
CD68, F4/80, CD4, CD117 |
CD68 |
|
CD4 |
CD68, CD66b, CD4 |
|
Read the blog post on the relationship between immunophenotyping & immunohistochemistry. [LINK COMING]
