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Histological stains (specialty)
The Hematoxylin and Eosin (H&E) method is the routine, recognized stain used within histology laboratories; however, many other special tinctorial stains are used on specific tissue or cellular components to aid diagnosis of toxicological or pathological changes.
Special Stain Method: |
Specialized Stain for the demonstration of: |
|---|---|
Alcian Blue |
Acid Mucins |
Alcian Blue PAS |
Acid, Neutral and Combined Mucins |
Aldehyde Fuchsin (Gomori’s) |
Lipofuscins |
Aldehyde Fuchsin (modified) |
Pancreatic Islet Cells |
Alizarin Red |
Calcium |
Carbol Chromotrope |
Eosinophil Leucocytes |
Chromotrope Aniline Blue |
Hyaline Droplets |
Congo Red |
Amyloid |
Dunn-Thompson |
Haemoglobin |
Ferric Hematoxylin |
Phospholipids |
Feulgen |
DNA |
Fluro Jade B (fluorescence) |
Degenerating Neurones |
Fouchet’s |
Bile Pigment |
Giemsa |
Parasites |
Glees Marsland (Bielschowsky) |
Central and Peripheral Nerves |
Goldner Trichrome |
Mineralised and Osteoid Bone |
Gordon and Sweets |
Reticulin |
Gram |
Bacteria |
Gridley’s |
Fungi |
Grimelius |
Argyrophil Cells |
Grocott’s (Gomori) |
Fungi and Basement Membranes |
Luxol Fast Blue and Cresyl Fast Violet |
Myelin and Nissl substance |
Martius Scarlet Blue |
Fibrin, Connective Tissue |
Masson Fontana |
Melanin |
Masson’s Trichrome |
Connective Tissue (Collagen) |
Methyl Green Pyronin |
DNA and RNA |
Movat’s Pentachrome |
Connective Tissue (Collagen) |
Nile Red (fluorescence) |
Lipids |
Okajima |
Haemoglobin |
Oil Red O |
Lipid |
Periodic Acid Schiff (PAS) Diastase |
Glycogen |
Periodic Acid Schiff (PAS) Orange G |
Acidophil Cells |
Periodic Acid Schiff (PAS) |
Periodate Carbohydrates, Basement Membranes |
Perl’s |
Ferric Iron |
Perl’s Prussian Blue |
Ferric Iron |
Phloxine Tartrazine |
Viral Inclusion Bodies |
PTAH (Phosophotungstic acid-haematoxylin) |
Muscle Striations |
Schmorl’s Ferricyanide |
Lipofuscin |
Sirius Red |
Amyloid and Eosinophils |
Solochrome Cyanin |
Myelin |
Toluidine Blue |
Mast Cells, Cartlidge Matrix, Acid Mucins |
Van Gieson (Picro-Fuchsin) |
Connective Tissue |
Verhoeff Van Gieson |
Elastic Fibres, Connective Tissue (Collagen) |
Von Kossa |
Mineralised Bone and Osteoid |
Warthin-Starry |
Spirochetes |
Ziehl-Neelsen |
Lipofuscin and Ceroid |
Ziehl-Neelsen (modified) |
Mycobacterium, TB, Leprae |
